SpiderImagePlugin.py 9.2 KB

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  1. #
  2. # The Python Imaging Library.
  3. #
  4. # SPIDER image file handling
  5. #
  6. # History:
  7. # 2004-08-02 Created BB
  8. # 2006-03-02 added save method
  9. # 2006-03-13 added support for stack images
  10. #
  11. # Copyright (c) 2004 by Health Research Inc. (HRI) RENSSELAER, NY 12144.
  12. # Copyright (c) 2004 by William Baxter.
  13. # Copyright (c) 2004 by Secret Labs AB.
  14. # Copyright (c) 2004 by Fredrik Lundh.
  15. #
  16. ##
  17. # Image plugin for the Spider image format. This format is used
  18. # by the SPIDER software, in processing image data from electron
  19. # microscopy and tomography.
  20. ##
  21. #
  22. # SpiderImagePlugin.py
  23. #
  24. # The Spider image format is used by SPIDER software, in processing
  25. # image data from electron microscopy and tomography.
  26. #
  27. # Spider home page:
  28. # https://spider.wadsworth.org/spider_doc/spider/docs/spider.html
  29. #
  30. # Details about the Spider image format:
  31. # https://spider.wadsworth.org/spider_doc/spider/docs/image_doc.html
  32. #
  33. from __future__ import annotations
  34. import os
  35. import struct
  36. import sys
  37. from . import Image, ImageFile
  38. def isInt(f):
  39. try:
  40. i = int(f)
  41. if f - i == 0:
  42. return 1
  43. else:
  44. return 0
  45. except (ValueError, OverflowError):
  46. return 0
  47. iforms = [1, 3, -11, -12, -21, -22]
  48. # There is no magic number to identify Spider files, so just check a
  49. # series of header locations to see if they have reasonable values.
  50. # Returns no. of bytes in the header, if it is a valid Spider header,
  51. # otherwise returns 0
  52. def isSpiderHeader(t):
  53. h = (99,) + t # add 1 value so can use spider header index start=1
  54. # header values 1,2,5,12,13,22,23 should be integers
  55. for i in [1, 2, 5, 12, 13, 22, 23]:
  56. if not isInt(h[i]):
  57. return 0
  58. # check iform
  59. iform = int(h[5])
  60. if iform not in iforms:
  61. return 0
  62. # check other header values
  63. labrec = int(h[13]) # no. records in file header
  64. labbyt = int(h[22]) # total no. of bytes in header
  65. lenbyt = int(h[23]) # record length in bytes
  66. if labbyt != (labrec * lenbyt):
  67. return 0
  68. # looks like a valid header
  69. return labbyt
  70. def isSpiderImage(filename):
  71. with open(filename, "rb") as fp:
  72. f = fp.read(92) # read 23 * 4 bytes
  73. t = struct.unpack(">23f", f) # try big-endian first
  74. hdrlen = isSpiderHeader(t)
  75. if hdrlen == 0:
  76. t = struct.unpack("<23f", f) # little-endian
  77. hdrlen = isSpiderHeader(t)
  78. return hdrlen
  79. class SpiderImageFile(ImageFile.ImageFile):
  80. format = "SPIDER"
  81. format_description = "Spider 2D image"
  82. _close_exclusive_fp_after_loading = False
  83. def _open(self):
  84. # check header
  85. n = 27 * 4 # read 27 float values
  86. f = self.fp.read(n)
  87. try:
  88. self.bigendian = 1
  89. t = struct.unpack(">27f", f) # try big-endian first
  90. hdrlen = isSpiderHeader(t)
  91. if hdrlen == 0:
  92. self.bigendian = 0
  93. t = struct.unpack("<27f", f) # little-endian
  94. hdrlen = isSpiderHeader(t)
  95. if hdrlen == 0:
  96. msg = "not a valid Spider file"
  97. raise SyntaxError(msg)
  98. except struct.error as e:
  99. msg = "not a valid Spider file"
  100. raise SyntaxError(msg) from e
  101. h = (99,) + t # add 1 value : spider header index starts at 1
  102. iform = int(h[5])
  103. if iform != 1:
  104. msg = "not a Spider 2D image"
  105. raise SyntaxError(msg)
  106. self._size = int(h[12]), int(h[2]) # size in pixels (width, height)
  107. self.istack = int(h[24])
  108. self.imgnumber = int(h[27])
  109. if self.istack == 0 and self.imgnumber == 0:
  110. # stk=0, img=0: a regular 2D image
  111. offset = hdrlen
  112. self._nimages = 1
  113. elif self.istack > 0 and self.imgnumber == 0:
  114. # stk>0, img=0: Opening the stack for the first time
  115. self.imgbytes = int(h[12]) * int(h[2]) * 4
  116. self.hdrlen = hdrlen
  117. self._nimages = int(h[26])
  118. # Point to the first image in the stack
  119. offset = hdrlen * 2
  120. self.imgnumber = 1
  121. elif self.istack == 0 and self.imgnumber > 0:
  122. # stk=0, img>0: an image within the stack
  123. offset = hdrlen + self.stkoffset
  124. self.istack = 2 # So Image knows it's still a stack
  125. else:
  126. msg = "inconsistent stack header values"
  127. raise SyntaxError(msg)
  128. if self.bigendian:
  129. self.rawmode = "F;32BF"
  130. else:
  131. self.rawmode = "F;32F"
  132. self._mode = "F"
  133. self.tile = [("raw", (0, 0) + self.size, offset, (self.rawmode, 0, 1))]
  134. self._fp = self.fp # FIXME: hack
  135. @property
  136. def n_frames(self):
  137. return self._nimages
  138. @property
  139. def is_animated(self):
  140. return self._nimages > 1
  141. # 1st image index is zero (although SPIDER imgnumber starts at 1)
  142. def tell(self):
  143. if self.imgnumber < 1:
  144. return 0
  145. else:
  146. return self.imgnumber - 1
  147. def seek(self, frame):
  148. if self.istack == 0:
  149. msg = "attempt to seek in a non-stack file"
  150. raise EOFError(msg)
  151. if not self._seek_check(frame):
  152. return
  153. self.stkoffset = self.hdrlen + frame * (self.hdrlen + self.imgbytes)
  154. self.fp = self._fp
  155. self.fp.seek(self.stkoffset)
  156. self._open()
  157. # returns a byte image after rescaling to 0..255
  158. def convert2byte(self, depth=255):
  159. (minimum, maximum) = self.getextrema()
  160. m = 1
  161. if maximum != minimum:
  162. m = depth / (maximum - minimum)
  163. b = -m * minimum
  164. return self.point(lambda i, m=m, b=b: i * m + b).convert("L")
  165. # returns a ImageTk.PhotoImage object, after rescaling to 0..255
  166. def tkPhotoImage(self):
  167. from . import ImageTk
  168. return ImageTk.PhotoImage(self.convert2byte(), palette=256)
  169. # --------------------------------------------------------------------
  170. # Image series
  171. # given a list of filenames, return a list of images
  172. def loadImageSeries(filelist=None):
  173. """create a list of :py:class:`~PIL.Image.Image` objects for use in a montage"""
  174. if filelist is None or len(filelist) < 1:
  175. return
  176. imglist = []
  177. for img in filelist:
  178. if not os.path.exists(img):
  179. print(f"unable to find {img}")
  180. continue
  181. try:
  182. with Image.open(img) as im:
  183. im = im.convert2byte()
  184. except Exception:
  185. if not isSpiderImage(img):
  186. print(img + " is not a Spider image file")
  187. continue
  188. im.info["filename"] = img
  189. imglist.append(im)
  190. return imglist
  191. # --------------------------------------------------------------------
  192. # For saving images in Spider format
  193. def makeSpiderHeader(im):
  194. nsam, nrow = im.size
  195. lenbyt = nsam * 4 # There are labrec records in the header
  196. labrec = int(1024 / lenbyt)
  197. if 1024 % lenbyt != 0:
  198. labrec += 1
  199. labbyt = labrec * lenbyt
  200. nvalues = int(labbyt / 4)
  201. if nvalues < 23:
  202. return []
  203. hdr = [0.0] * nvalues
  204. # NB these are Fortran indices
  205. hdr[1] = 1.0 # nslice (=1 for an image)
  206. hdr[2] = float(nrow) # number of rows per slice
  207. hdr[3] = float(nrow) # number of records in the image
  208. hdr[5] = 1.0 # iform for 2D image
  209. hdr[12] = float(nsam) # number of pixels per line
  210. hdr[13] = float(labrec) # number of records in file header
  211. hdr[22] = float(labbyt) # total number of bytes in header
  212. hdr[23] = float(lenbyt) # record length in bytes
  213. # adjust for Fortran indexing
  214. hdr = hdr[1:]
  215. hdr.append(0.0)
  216. # pack binary data into a string
  217. return [struct.pack("f", v) for v in hdr]
  218. def _save(im, fp, filename):
  219. if im.mode[0] != "F":
  220. im = im.convert("F")
  221. hdr = makeSpiderHeader(im)
  222. if len(hdr) < 256:
  223. msg = "Error creating Spider header"
  224. raise OSError(msg)
  225. # write the SPIDER header
  226. fp.writelines(hdr)
  227. rawmode = "F;32NF" # 32-bit native floating point
  228. ImageFile._save(im, fp, [("raw", (0, 0) + im.size, 0, (rawmode, 0, 1))])
  229. def _save_spider(im, fp, filename):
  230. # get the filename extension and register it with Image
  231. ext = os.path.splitext(filename)[1]
  232. Image.register_extension(SpiderImageFile.format, ext)
  233. _save(im, fp, filename)
  234. # --------------------------------------------------------------------
  235. Image.register_open(SpiderImageFile.format, SpiderImageFile)
  236. Image.register_save(SpiderImageFile.format, _save_spider)
  237. if __name__ == "__main__":
  238. if len(sys.argv) < 2:
  239. print("Syntax: python3 SpiderImagePlugin.py [infile] [outfile]")
  240. sys.exit()
  241. filename = sys.argv[1]
  242. if not isSpiderImage(filename):
  243. print("input image must be in Spider format")
  244. sys.exit()
  245. with Image.open(filename) as im:
  246. print("image: " + str(im))
  247. print("format: " + str(im.format))
  248. print("size: " + str(im.size))
  249. print("mode: " + str(im.mode))
  250. print("max, min: ", end=" ")
  251. print(im.getextrema())
  252. if len(sys.argv) > 2:
  253. outfile = sys.argv[2]
  254. # perform some image operation
  255. im = im.transpose(Image.Transpose.FLIP_LEFT_RIGHT)
  256. print(
  257. f"saving a flipped version of {os.path.basename(filename)} "
  258. f"as {outfile} "
  259. )
  260. im.save(outfile, SpiderImageFile.format)